…these events underlie a switch to expression of short mRNA isoforms and preferential use of alternative last exons in a number of genes, including ASCC3. Intriguingly, the switch between ASCC3 isoforms occurs on more than one level, in that the long mRNA isoform encodes a protein, functioning in the context of the ASCC complex and required for maintaining transcriptional suppression in the late stages of the DNA damage response, whereas the short isoform functions as a nuclear non-coding RNA that is required for transcription to recover. Intriguingly, the short and long isoforms constitute an autonomous regulatory module and functionally interrelate, so that the effect of deleting one can be at least partially compensated for by deleting the other (Figure 7H).
There is no such thing as an autonomous regulatory module. All RNA-mediated protein folding chemistry is energy dependent and functional interrelations link differences in the energy of photons from natural selection for energy-dependent codon optimality to all biodiversity on Earth via the physiology of reproduction.
Reported as: Noncoding RNA Helps Cells Recover from DNA Damage
“If the protein is made from the long pre-mRNA, then global transcription is repressed. But if the short RNA is made, it helps recover transcription hours after damage.”
How the short isoform aids repair remains unknown.
My comment to The Scientist: There is clear evidence that femtosecond blasts of UV light repair DNA in the context of energy-dependent changes in the microRNA/messenger RNA balance and autophagy, which protects all organized genomes from virus-driven energy theft and the degradation of messenger RNA.
The failure to integrate the Nobel Prize winning works of Ben Feringa (Chemisty 2016) and Yoshinori Ohsumi (Physiology or Medicine 2016) prevents theorists from linking what organisms eat to their pheromone-controlled physiology of reproduction in the context of Schrodinger’s claims in “What is Life?”(1944) and this claim by Roger Penrose in the reprint edition:
“How often do we still hear that quantum effects can have little relevance in the study of biology, or even that we eat food in order to gain energy?” (Roger Penrose 8 August 1991)
See also: From Fertilization to Adult Sexual Behavior In our section on molecular epigenetics, we wrote:
Small intranuclear proteins also participate in generating alternative splicing techniques of pre-mRNA and, by this mechanism, contribute to sexual differentiation in at least two species, Drosophila melanogaster and Caenorhabditis elegans…
The food energy that is linked from alternative splicing techniques of pre-mRNA to the chemistry of protein folding and the pheromone-controlled physiology of reproduction in all living genera seems to be largely ignored by those who are not Nobel Laureates.